Exposure of rheumatoid arthritis fibroblast-like synoviocytes to 0.5% DMSO resulted in ≈25% cell death after 24 hours, indicating concentrations must be below 0.05% to be considered safe.
What is the safe concentration of DMSO for human fibroblast-like synoviocytes from rheumatoid arthritis patients?
DMSO exhibits significant cytotoxicity in human fibroblast-like synoviocytes at concentrations as low as 0.5%, suggesting that concentrations below 0.05% should be used to ensure safety in in vitro studies.
The effect of dimethyl sulfoxide (DMSO) in rheumatoid arthritis (RA) human fibroblast-like synoviocytes (FLSs) has been studied on five different samples harvested from the joints (fingers, hands and pelvis) of five women with RA. At high concentrations (>5%), the presence of DMSO induces the cleavage of caspase-3 and PARP-1, two phenomena associated with the cell death mechanism. Even at a 0.5% concentration of DMSO, MTT assays show a strong toxicity after 24 h exposure (≈25% cell death). Therefore, to ensure a minimum impact of DMSO on RA FLSs, our study shows that the concentration of DMSO has to be below 0.05% to be considered safe.
Gallardo‐Villagrán et al. (Wed,) conducted a other in Rheumatoid arthritis (n=5). Dimethyl sulfoxide (DMSO) was evaluated on Cell death (toxicity via MTT assay). Exposure of rheumatoid arthritis fibroblast-like synoviocytes to 0.5% DMSO resulted in ≈25% cell death after 24 hours, indicating concentrations must be below 0.05% to be considered safe.
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