What is the clinical evidence from this study?

Population: Rat ventricular muscle (n=11). Intervention: Skinning with Triton X-100 vs. Intact muscle. Primary outcome: Force-[Ca2+] relation (K1/2 for half-maximal activation) (p=< .05).

What question did this study set out to answer?

This study aims to compare the Ca2+ sensitivity of myofilaments in intact and skinned rat ventricular muscle.

March 1, 1994Circulation ResearchOpen Access

Myofilament Ca2+ sensitivity in intact versus skinned rat ventricular muscle.

Q: What are the key findings of this study?

Skinning of rat ventricular trabeculae significantly altered myofilament Ca2+ responsiveness, shifting the force-[Ca2+] relation rightward compared to intact muscle (P < .05).

Q: What question did this study set out to answer?

This study aims to compare the Ca2+ sensitivity of myofilaments in intact and skinned rat ventricular muscle.

View Full Paper

Ask AI

Bookmark

Key result

Skinning of rat ventricular trabeculae significantly altered myofilament Ca2+ responsiveness, shifting the force-[Ca2+] relation rightward compared to intact muscle (P < .05).

Key Points

This study aims to compare the Ca2+ sensitivity of myofilaments in intact and skinned rat ventricular muscle.
Trabeculae from rat right ventricle were loaded with fura-2 and stimulated at 10 Hz.
Intact force-Ca2+ relation measured with and without skinning using Triton X-100.
Ca2+ sensitivity was quantified using the Hill equation and K1/2 values.
Intact muscle had a Hill coefficient of 4.87 and K1/2 of 0.62 mumol/L.
Post-skinning, the Hill coefficient decreased to 2.72 and K1/2 increased to 2.2 mumol/L.
Alterations in myofilament responsiveness were observed even under varying Mg2+ concentrations.

Structured PICO

Population

Trabeculae from rat right ventricle

Intervention

Skinning with Triton X-100 (1%)

Comparator

Intact muscle (before skinning)

Outcome

Force-[Ca2+] relation (Hill coefficient and K1/2)surrogate

Main Result

Absolute Event Rate: 0.93% vs 0.62%

p-value: p=< .05

Skinning of rat ventricular muscle alters the Ca2+ responsiveness of myofilaments compared to intact muscle, even when accounting for intracellular Mg2+ levels.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Skinned preparations may not reflect intact myofilament Ca2+ sensitivity; leaves open optimal methods for translational myofilament research.

Cite This Study

Gao et al. (1994) studied Rat ventricular muscle (n=11). Skinning with Triton X-100 vs. Intact muscle was evaluated on Force-[Ca2+] relation (K1/2 for half-maximal activation) (p=< .05). Skinning of rat ventricular trabeculae significantly altered myofilament Ca2+ responsiveness, shifting the force-[Ca2+] relation rightward compared to intact muscle (P < .05).

synapsesocial.com/papers/6a0f7a9fd8c5cf602efcc289 https://doi.org/10.1161/01.res.74.3.408

View Full Paper

Ask AI

Bookmark

Implication

Skinned preparations may not reflect intact myofilament Ca2+ sensitivity; leaves open optimal methods for translational myofilament research.

Myofilament Ca2+ sensitivity in intact versus skinned rat ventricular muscle.

Key Points

Structured PICO

Main Result

Discussion

Implication

Cite This Study

Myofilament Ca2+ sensitivity in intact versus skinned rat ventricular muscle.

Key Points

Structured PICO

Main Result

Discussion

Implication

Cite This Study

Also Consider

Also Consider