Inhibition of RGMa attenuates neuronal autophagy and improves neurological function in a rat ischemia/reperfusion model

BACKGROUND: Ischemic stroke (IS) is a leading global cause of morbidity and mortality, with neuronal autophagy playing a pivotal role in ischemia/reperfusion (I/R) injury following stroke. Repulsive guidance molecule A (RGMa), known for its involvement in axon guidance and neuronal repair, has recently been linked to I/R injury. This study aimed to explore RGMa's role in neuronal autophagy and its therapeutic potential in mitigating I/R-induced damage using a rat model. METHODS: = 3-4) were evaluated using 2,3,5-triphenyl tetrazolium chloride (TTC) staining, behavioral scores, immunofluorescence, Western blotting, and transmission electron microscopy (TEM). RGMa's autophagy mechanism was assessed by co-immunoprecipitation. RESULTS: = 3) in treated groups. RGMa did not interact with Beclin1-PIK3C3, suggesting a Beclin1-independent pathway.