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January 1, 2026RSC AdvancesOpen Access

NanoBRET-based detection of ligand–receptor interactions at the neuropeptide FF receptor 1

Hannah Lentschat Annette G. Beck‐Sickinger

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Key Points

The aim is to characterize ligand binding at the neuropeptide FF receptor 1 using the nanoBRET assay.
Utilized a nanoBRET-based assay for detecting ligand-receptor interactions.
Characterized binding properties of ligands at NPFFR1.
Assessed signal output to evaluate binding efficacy.
Demonstrated effective ligand binding at NPFFR1 with clear signal specificity.
Provided insights into the dynamics of receptor interactions.
Highlighted potential applications in drug discovery in neuromodulatory systems.

Implication

Demonstrates ligand binding at neuropeptide FF receptor 1, highlighting molecular interactions.

Abstract

Characterization of ligand binding at the neuropeptide FF receptor 1 (NPFFR1) through a nanoBRET-based assay.

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Cite This Study

Lentschat et al. (2026) studied this question.

synapsesocial.com/papers/6a0ff374d674f7c03778c12f https://doi.org/10.1039/d6ra01350c

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