Brevundimonas sediminis sp. nov., isolated from mangrove sediment

Two Gram-stain-negative, facultatively anaerobic, light-yellow, rod-shaped bacterial strains, designated as FT23028 T and FT23042, were isolated from mangrove sediment collected in Shenzhen, PR China. Their taxonomic positions were established using a polyphasic approach including phylogenetic, physiological, biochemical and chemotaxonomic analyses. Both strains grew optimally at 25–30 °C and pH 7.0 without NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains FT23028 T and FT23042 were most closely related to Brevundimonas kwangchunensis KCTC 12380 T (98.31 and 98.23%), Brevundimonas lenta KCTC 12871 T (98.10 and 98.03%) and Brevundimonas viscosa CGMCC 1.10683 T (97.68 and 97.61%), respectively. These two strains shared 99.93% 16S rRNA gene sequence similarity with each other. The predominant fatty acids (>10%) identified in strains FT23028 T and FT23042 were C 18:1 ω 7c (and/or C 18:1 ω 6c, 48.7 and 53.2%, respectively) and C 16:0 (29.9 and 26.4%, respectively). The polar lipid profiles included two unknown phosphoglycolipids, five glycolipids and three unidentified lipids. The respiratory quinone was Q-10. The total sequence lengths of strains FT23028 T and FT23042 were 3,122,877 and 3,235,296 bp, with DNA G+C contents of 65.3 and 67.4 mol%, respectively. Based on phenotypic, chemotaxonomic and phylogenetic distinction, a novel species of the genus Brevundimonas is proposed, for which the name Brevundimonas sediminis sp. nov. is suggested. The type strain is FT23028 T (=MCCC 1K09458 T =KCTC 8783 T ).