Abstract: Feruloyl esterase production by Aspergillus oryzae was investigated using plant fibre materials as substrates. The fibre samples (Sugarcane bagasse and brans of wheat, rice, maize, and barley) were purchased from retailers at Lafenwa market in Abeokuta, Ogun state. They were allowed to decay spontaneously for the isolation of Aspergillus oryzae. The fungus (Aspergillus oryzae) was isolated and characterised using phenotypic and molecular techniques and screened for production of feruloyl esterase using all the fibre materials as substrate. Optimum conditions for feruloyl esterase production by Aspergillus oryzae was determined with standard methods using all the bran as substrate. The inoculum size for feruloyl esterase production was 1.40 x104 spores/ml and the enzyme produced ranged from 0.48 U/mL with rice-bran and maize-bran to 1.26 U/mL with wheat-bran. The optimum temperature, pH, time of incubation and inoculum concentration for the production of feruloyl esterase by the fungus using wheat-bran as substrate were found to be 30oC, 7.0, 120hrs and 1.0 respectively. Keywords: Aspergillus oryzae, feruloyl esterase, fibre materials, optimum conditions. Title: FERULOYL ESTERASE PRODUCTION DURING DEGRADATION OF DIFFERENT FIBRES BY Aspergillus oryzae Author: Jiboku Olukemi O., Ewuoso Lateefat M., Yusuff Kareem O., Ibitokun Oluwatoyin R. International Journal of Novel Research in Life Sciences ISSN 2394-966X Vol. 12, Issue 5, September 2025 – October 2025 Page No: 1-7 Novelty Journals Website: www.noveltyjournals.com Published Date: 14-October-2025 DOI: https://doi.org/10.5281/zenodo.17348824 Paper Download Link (Source) https://www.noveltyjournals.com/upload/paper/FERULOYL%20ESTERASE%20PRODUCTION%20DURING-14102025-4.pdf
O. et al. (Tue,) studied this question.