Key result
Exposure to a single 15 Gy dose of ionizing radiation in rats led to increased collagen deposition, elevated caspase-3-cleaved levels, and reduced ejection fraction at 13 months.
Why the study?
Does a single dose of radiation (15 Gy) to the heart induce caspase-3 activation, collagen deposition, and ventricular function decline in female Wistar rats?
Does a single dose of radiation (15 Gy) to the heart induce caspase-3 activation, collagen deposition, and ventricular function decline in female Wistar rats?
Radiation-induced decline in cardiac function is associated with increased collagen deposition and caspase-3 activation.
May caution against high-dose cardiac irradiation; leaves open translation to human radiotherapy outcomes.
The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and functional (reduced ejection fraction) were observed. Data suggest that the cardiac function decline after exposure to ionizing radiation is related, in part, to increased collagen and increased caspase-3-cleaved.
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Ferreira-Machado et al. (2013) studied Radiation-induced cardiac dysfunction. Ionizing radiation vs. Non-irradiated control was evaluated on Levels of procollagen type I, TGF-ß1, and caspase-3-cleaved, and cardiac structural/functional alterations. Exposure to a single 15 Gy dose of ionizing radiation in rats led to increased collagen deposition, elevated caspase-3-cleaved levels, and reduced ejection fraction at 13 months.
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