What question did this study set out to answer?

To investigate the factors determining the charge-state distribution of gramicidin S cations electrosprayed from basic solutions containing various nitrogen bases.

November 1, 1994Organic Mass Spectrometry

Electrospray mass spectra of protein cations formed in basic solutions

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Authors

JBJ. C. Yves Le BlancSciex (Canada)JWJianyao WangJohnson & Johnson (United States)RGRoger GuevremontPacific Northwest National Laboratory

Discussion

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Implication

Experimental mass spectrometry study reveals that peptide cation charge states correlate with base proton affinity rather than pH, highlighting gas-phase adduct dissociation mechanisms.

Key Points

To investigate the factors determining the charge-state distribution of gramicidin S cations electrosprayed from basic solutions containing various nitrogen bases.
Analyzed electrospray mass spectra of gramicidin S cations prepared in 0.2 M solutions across 18 nitrogen-containing bases.
Evaluated ion distributions across collision regions, including measurements performed under nominally zero collision energy conditions.
Relative abundances of [M + 2H]²⁺ to [M + H]⁺ ions correlated with the proton affinities of the basic additives rather than the bulk solution pH.
Nitrogen-base adducts desorbed into the gas phase and underwent collision-induced dissociation in the lens region, with intact adducts directly observed at zero collision energy.

Cite This Study

Blanc et al. (1994) studied this question.

synapsesocial.com/papers/6a10f9a263b25c787d9fd232 https://doi.org/10.1002/oms.1210291103

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November 1, 1994Organic Mass Spectrometry

Electrospray mass spectra of protein cations formed in basic solutions

View Full Paper

Ask AI

Bookmark

Authors

JBJ. C. Yves Le BlancSciex (Canada)JWJianyao WangJohnson & Johnson (United States)RGRoger GuevremontPacific Northwest National Laboratory

Discussion

Member takes

Implication

Experimental mass spectrometry study reveals that peptide cation charge states correlate with base proton affinity rather than pH, highlighting gas-phase adduct dissociation mechanisms.

Key Points

To investigate the factors determining the charge-state distribution of gramicidin S cations electrosprayed from basic solutions containing various nitrogen bases.
Analyzed electrospray mass spectra of gramicidin S cations prepared in 0.2 M solutions across 18 nitrogen-containing bases.
Evaluated ion distributions across collision regions, including measurements performed under nominally zero collision energy conditions.
Relative abundances of [M + 2H]²⁺ to [M + H]⁺ ions correlated with the proton affinities of the basic additives rather than the bulk solution pH.
Nitrogen-base adducts desorbed into the gas phase and underwent collision-induced dissociation in the lens region, with intact adducts directly observed at zero collision energy.

Cite This Study

Blanc et al. (1994) studied this question.

synapsesocial.com/papers/6a10f9a263b25c787d9fd232 https://doi.org/10.1002/oms.1210291103

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