Visualizing increased uptake of [18F]FDG and [18F]FTHA in kidneys from obese high-fat diet fed C57BL/6J mice using PET/CT ex vivo

Figures

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  Fig 1. In vivo vs ex vivo PET/CT of mouse kidney. Old mice on chow diet, ad libitum fed or fasted (n = 4–5). (A) FDG SUV values for in vivo and ex vivo scans, (B) a representative in vivo scan of a fasted mouse and (C) ex vivo scan of organs from the same mouse. Data are presented as individual values. Students paired t-test. **p<0.01.

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  Fig 2. Effects on uptake of FDG or FTHA in mouse kidney by HFD or age. (A) Mean value of SUV in kidneys from ad libitum fed (n = 6–8) and (B) fasted mice (n = 6–7) injected with FDG. (C) Representative images of FDG uptake in kidney (both kidneys from the same individual). (D) Mean values of SUV in kidneys from ad libitum fed (n = 3–4) and (E) fasted (n = 3–4) mice injected with FTHA. (F) Representative images of FTHA uptake in kidney. Chow fed mice were either young (3 months old) or old (8 months), and high fat diet fed mice (HFD) were given HFD for 5 months starting from 3 months of age. Within each diet group, mice were either ad libitum fed or fasted (4 hours) before i.v. injection of FDG or FTHA. Data are presented as mean ± SD. One-Way ANOVA, Tukey´s post hoc test; *p<0.05. (dx = dexter; sin = sinister).

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  Fig 3. Effects on uptake of FDG and FTHA in mouse heart by HFD or age. Same mice as in Fig 2. (A) Mean value of SUV in heart from ad libitum fed (n = 6–9) and (B) fasted mice (n = 6–7) injected with FDG. (C) Mean values of SUV in heart from ad libitum fed (n = 4) and (D) fasted (n = 3–4) mice injected with FTHA. Chow fed mice were either young (3 months old) or old (8 months), and high fat diet fed mice (HFD) were given HFD for 5 months starting from 3 months of age. Within each diet group, mice were either ad libitum fed or fasted (4 hours) before i.v. injection of FDG or FTHA.Data are presented as mean ± SD. One-Way ANOVA, Tukey´s post hoc test or Students unpaired t-test; **p<0.01.

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  Fig 4. Triglyceride levels in mouse kidney and heart. Triglyceride (TG) (μg TG per mg wet tissue) was measured in kidneys from (A) ad libitum fed mice and (B) fasted mice (4+3 hours). TG in hearts from (C) ad libitum fed mice and (D) fasted mice (4+3 hours). Chow fed mice were either young (3 months old) or old (8 months), and high fat diet fed mice (HFD) were given HFD for 5 months from 3 months of age. Data are presented as mean ± SD. One-Way ANOVA with Tukey´s post hoc test; *p<0.05, **p<0.001.

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  Fig 5. Effects of HFD and age on the nutritional regulation of LPL activity. LPL activity was measured in fasted (4+3 hours) and ad libitum fed mice (n = 6–8). (A) LPL activity in perigonadal white adipose tissue (pgWAT) is presented as mU/mg total protein. (B) LPL activity in kidney, expressed as mU/g wet tissue. (C) LPL activity in heart as mU/g wet tissue. (D) mRNA expression of Angptl4 in kidneys, relative to Rn18s. Chow fed mice were either young (3 months old) or old (8 months), and high fat diet fed mice (HFD) were given HFD for 5 months from 3 months of age. Data are presented as mean ± SD. Students unpaired t-test; *p<0.05, **p<0.01, ****p<0.0001.