The role of (¹⁸F)-fluoro-D-glucose positron emission tomography/computed tomography in the surveillance of abnormal myocardial energy metabolism and cardiac dysfunction in a rat model of cardiopulmonary resuscitation

Figures

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  Figure 1. (a-d) Energy production and function are significantly impaired in the heart after cardiopulmonary resuscitation (CPR). (a) Expression levels of cardiac troponin I in the hearts of wild-type rats was detected with Assay Kit I after sham, CPR, and trimetazidine (TMZ) + CPR treatment for 6 hours (n = 4–5 rats per experimental group, #P < 0.05 vs. CPR 6 hours); (b) the results of the multiple reaction monitoring approach showed the levels of energy metabolism marker adenosine triphosphate in wild-type rats after TMZ + CPR, CPR, and sham treatment for 6 hours (n = 4–5 rats per experimental group, #P < 0.05 vs. CPR 6 hours); (c, d) Statistical results for the parameters of the echocardiographic results, including ejection fraction and fraction shortening in wild-type rats after sham surgery, CPR, and TMZ + CPR for 6 or 24 hours (n = 3 rats per experimental group, #P < 0.05 vs. CPR 24 hours).

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  Figure 2. (a-f) Glucose uptake and abnormal glycolysis are significantly enhanced in the animal heart after cardiopulmonary resuscitation (CPR). (a, b) Expression levels of phosphoenolpyruvate and 3-phospho-D-glycerate were increased in the hearts of wild-type rats, detected with the multiple reaction monitoring (MRM) approach after sham, CPR, and trimetazidine (TMZ) + CPR surgery for 6 hours (n = 4–5 rats per experimental group, #P < 0.05 vs. CPR 6 hours); (c) the lactate/pyruvate ratio was detected after sham, CPR, and TMZ + CPR surgery for 6 hours. (n = 4–5 rats per experimental group, #P < 0.05 vs. CPR 6 hours); (df) The results of the MRM approach showed the expression of alpha-ketoglutarate, succinate, and citrate in Sprague-Dawley rats’ hearts after sham, CPR, and TMZ + CPR treatment for 6 hours. (n = 4–5 rats per experimental group, #P < 0.05 vs. CPR 6 hours).

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  Figure 3. (a, b) (18F)-fluoro-D-glucose (FDG) positron emission tomography/computed tomography [(18F)-FDG PET/CT] can monitor the changes in cardiac function and myocardial energy metabolism after cardiopulmonary resuscitation (CPR) by tracking glucose metabolism. (a) (18F)-FDG PET/CT imaging showed FDG uptake after sham, CPR, and trimetazidine (TMZ) + CPR treatment for 6 hours (n = 3 rats per experimental group, #P < 0.05 vs. CPR 6 hours); (b) Statistical results for the value of standardized uptake value in the hearts of TMZ-treated wild-type rats after CPR or sham treatment for 6 hours (n = 3 rats per experimental group, #P < 0.05 vs. CPR 6 hours).