What question did this study set out to answer?

To determine whether electroporation can deliver CRISPR/Cas9 components into mouse zygotes to achieve targeted genome editing without requiring manual microinjection.

March 27, 2015GeneticsOpen Access

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

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Authors

WQWenning QinElectrical Geodesics (United States)SDStephanie DionJackson Laboratory PKPeter M. KutnyJackson Laboratory

Discussion

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Implication

Experimental study demonstrates efficient CRISPR/Cas9 gene editing via zygote electroporation in mice, indicating a high-throughput alternative to microinjection.

Key Points

To determine whether electroporation can deliver CRISPR/Cas9 components into mouse zygotes to achieve targeted genome editing without requiring manual microinjection.
Delivered Cas9 messenger RNA, single-guide RNA, and donor oligonucleotides into mouse zygotes via electroporation.
Assayed live-born mice for targeted nonhomologous end joining and homology-directed repair mutations.
Electroporation effectively introduced all necessary CRISPR/Cas9 editing components into mouse zygotes simultaneously.
Live mice carrying both nonhomologous end joining and homology-directed repair mutations were generated with high efficiency.

Cite This Study

Qin et al. (2015) studied this question.

synapsesocial.com/papers/6a11cfa71d1aaf8555563567 https://doi.org/10.1534/genetics.115.176594

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March 27, 2015GeneticsOpen Access

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

View Full Paper

Ask AI

Bookmark

Authors

WQWenning QinElectrical Geodesics (United States)SDStephanie DionJackson Laboratory PKPeter M. KutnyJackson Laboratory

Discussion

Member takes

Implication

Experimental study demonstrates efficient CRISPR/Cas9 gene editing via zygote electroporation in mice, indicating a high-throughput alternative to microinjection.

Key Points

To determine whether electroporation can deliver CRISPR/Cas9 components into mouse zygotes to achieve targeted genome editing without requiring manual microinjection.
Delivered Cas9 messenger RNA, single-guide RNA, and donor oligonucleotides into mouse zygotes via electroporation.
Assayed live-born mice for targeted nonhomologous end joining and homology-directed repair mutations.
Electroporation effectively introduced all necessary CRISPR/Cas9 editing components into mouse zygotes simultaneously.
Live mice carrying both nonhomologous end joining and homology-directed repair mutations were generated with high efficiency.

Cite This Study

Qin et al. (2015) studied this question.

synapsesocial.com/papers/6a11cfa71d1aaf8555563567 https://doi.org/10.1534/genetics.115.176594

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