According to the fact that the H component in HSI color space contains most of the white blood cell information, and the S component contains the structure information of the white blood cell nucleus, we develop an iterative Otsu's approach based on circular histogram for the leukocyte segmentation by taking full advantage of this knowledge. Experimental results show that this method works successfully in the segmentation of color smear microscopic images
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Wu et al. (2006) studied this question.
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