Relationship Between CASA Sperm Motility Parameters and Oxidative-Stress-Related Protein Markers in Common Carp (Cyprinus carpio)

The assessment of sperm quality is essential for improving reproductive efficiency in aquaculture species such as common carp (Cyprinus carpio). While sperm motility is widely used as a key functional indicator, the predictive value of oxidative protein markers for sperm performance remains unclear. In this study, sperm motility and kinematic parameters were assessed using computer-assisted sperm analysis (CASA). Markers of oxidative status, advanced oxidation protein products (AOPPs), ceruloplasmin (CP), and total protein concentration were quantified in samples. The results showed relatively high total motility (70.51%) and progressive motility (69.00%), indicating good sperm quality. No statistically significant correlations (p > 0.0.5) were found between AOPP or CP and sperm motility. Protein concentration was positively associated with motility parameters, including curvilinear velocity (VCL) and amplitude of lateral head displacement (ALH). A significant negative correlation was found between Fulton’s condition factor (FCF) and AOPP (r = −0.686; p < 0.05), indicating that individuals with higher body condition had lower oxidative protein damage. These findings suggest that oxidative protein markers may not directly reflect sperm motility, highlighting the potential value of including additional oxidative stress-related parameters in sperm quality assessment.