Genome-Wide Identification, Characterization and Expression Profiling of myosin Family Genes in Sebastes schlegelii

Figures

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  Figure 1. The phylogenetic analysis and classification of myosin genes. (A) Phylogenetic tree of myosin genes was constructed with Neighbor-Joining method by MEGA7 (bootstrap = 1000). Twelve subfamilies were decorated by different colors. (B) Characterization of Myo2 subfamily in teleost fishes. The phylogenetic tree was conducted by MEGA 7.0 using CO1 sequences of these species. The dotted border represented gene loss in the species.

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  Figure 2. A schematic diagram of S. schlegelii myosin gene domains. The maximum likelihood method was used to construct the phylogenetic tree of myosin genes (left panel). The rectangle with different colors represented different domains (right panel). The scale bar indicated 500 amino acid residues.

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  Figure 3. Gene structure and motif analysis of myosin genes. The maximum likelihood method was used to conduct the phylogenetic analysis of myosin genes (left panel). The exons and UTRs were indicated by orange and blue boxes (the middle panel) respectively. Different putative motifs were indicated by boxes with different colors (right panel).

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  Figure 4. Chromosome location and synteny analysis of myosin genes. The boxes with different colors represented different chromosomes of S. schlegelii. The red lines represented the syntenic relationships of myosin genes in S. schlegelii.

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  Figure 5. Expression profiles of myosin genes in adult tissues (A) and early developmental stages (B). The color scale represented the TPM (Transcripts-per-million) value. The red and blue color represented the relatively higher and lower TPM (Transcripts-per-million) value, respectively.

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  Figure 6. Myo2 genes participated in S. schlegelii muscle growth. (A, C) H&E staining showed the process of muscle growth in juvenile and adult S. schlegelii. (B) Relative expression of five Myo2 genes during different stages of muscle growth in juvenile fish. Data are shown as mean ± SEM (n = 3). Different letters indicated statistical significance (p < 0.05). (D) Relative expression of five Myo2 genes in adult (1.5 and 2.5-year-old) S. schlegelii. Data are shown as mean ± SEM (n = 3). Marks * and ** indicated statistical significance (p < 0.05 or p < 0.01).

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  Figure 7. The expression patterns of myosin genes in muscle cell lines at different time points after differentiation induction. The color scale represented the TPM (transcripts-per-million) value. The red and blue color represented the relatively higher and lower TPM (transcripts-per-million) value, respectively.