A combination of plasma EV-derived miR-146b-5p, miR-4701-3p, and miR-1180-3p demonstrated high discriminative capacity for subclinical coronary atherosclerosis (AUC = 0.8281, sensitivity = 93.75%).
Observational
Does a plasma EV-derived miRNA signature accurately identify subclinical coronary atherosclerosis in asymptomatic individuals?
A specific plasma EV-derived miRNA signature shows high discriminative capacity for detecting subclinical coronary atherosclerosis, suggesting potential as a non-invasive biomarker for early risk assessment.
Effect estimate: AUC 0.8281
Subclinical coronary atherosclerosis (SCA) is an early stage of coronary artery disease (CAD) that often goes unrecognized until clinical events occur. Identifying circulating molecular biomarkers could improve early diagnosis and risk assessment in asymptomatic individuals. This study employed a two-phase approach to identify plasma extracellular vesicle (EV)-derived microRNAs (miRNAs) associated with SCA. In the discovery phase, plasma samples from male participants were analyzed using Affymetrix GeneChip miRNA 4.0 microarrays. Differentially expressed miRNAs were refined through bioinformatic analysis, cross-species comparison with murine data, and target gene prediction. In the validation phase, six candidate miRNAs were quantified by RT-qPCR in an independent cohort. Six miRNAs were differentially expressed between individuals with SCA and controls. Among these, the combination of miR-146b-5p, miR-4701-3p, and miR-1180-3p demonstrated a high discriminative capacity for SCA (AUC = 0.8281; sensitivity = 93.75%; specificity = 93.75%). Functional enrichment analysis revealed that predicted target genes are involved in key atherosclerosis-related pathways, including inflammation, lipid metabolism, and vascular remodeling. EV-derived miRNAs may serve as non-invasive biomarkers for the early detection of coronary atherosclerosis. These findings provide insight into the molecular processes underlying subclinical vascular disease and support the integration of EV-associated miRNAs into preventive cardiology strategies.
Peña-Peña et al. (Sun,) conducted a observational in Subclinical coronary atherosclerosis (SCA). Plasma extracellular vesicle-derived microRNA signature (miR-146b-5p, miR-4701-3p, miR-1180-3p) vs. Controls was evaluated on Discriminative capacity for subclinical coronary atherosclerosis (AUC 0.8281). A combination of plasma EV-derived miR-146b-5p, miR-4701-3p, and miR-1180-3p demonstrated high discriminative capacity for subclinical coronary atherosclerosis (AUC = 0.8281, sensitivity = 93.75%).
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