Aldosterone antibodies were produced by injecting sheep with the albumin conjugate of aldosterone-18,21-dihemisuccinate. Conditions optimal for an accurate, reproducible assay with this antiserum were determined experimentally. Aldosterone is extracted from plasma and isolated by a single thin-layer chromatographic system before the immunoassay is performed. Normal plasma aldosterone in supine subjects is 8.6 ± 3.8 ng/100 ml; after 2 h in the upright position, 18.3 ± 9.5 ng/100 ml. Eighteen months of experience with the method have proved it to be reliable with respect to reproducibility, accuracy, and precision.
No takes yet. Share an insight, caveat, or question.
Cyr et al. (1972) studied this question.