Key result
An automated optical mapping system using voltage-sensitive fluorescent dyes reliably describes activation sequences and quantifies conduction velocities in isolated murine atrial tissue.
An automated optical mapping system using voltage-sensitive dyes enables reliable, high-throughput quantification of electrophysiological parameters in murine atrial tissue.
Does not inform clinical care; extends automated optical mapping for murine atrial research.
Optical mapping is an important tool for assessment of cardiac electrophysiology. We demonstrate a system for quantification and automatic measurement of electrophysiological parameters in isolated cardiac tissue. The system makes use of voltage sensitive fluorescent dyes. These shift in wavelength in response to millivolt changes in potential across cell membranes located on the left atrium. Automated analysis of the pixel-wise measurements yields information on action potential durations and isochronal maps allowing for high throughput of data analysis. The algorithms that we propose reliably describe activation sequences and allow for quantification of conduction velocities.
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Yu et al. (2015) studied Murine atrial electrophysiology. Optical mapping system was evaluated on Action potential durations and conduction velocities. An automated optical mapping system using voltage-sensitive fluorescent dyes reliably describes activation sequences and quantifies conduction velocities in isolated murine atrial tissue.
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