What does this research mean for the field?

Canine cardiac phospholamban is a 52-amino acid protein with an inferred molecular weight of 6,080, consisting of a hydrophilic cytoplasmic domain with phosphorylatable sites and a hydrophobic membrane-anchored domain. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.SUPPORTS_CONSENSUS.

What question did this study set out to answer?

This research aims to elucidate the complete amino acid sequence of canine cardiac phospholamban derived from cDNA.

January 1, 1987Journal of Clinical InvestigationOpen Access

Complete complementary DNA-derived amino acid sequence of canine cardiac phospholamban.

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Population

Canine cardiac ventricular muscle from adult dog

Design

Preclinical

Authors

JFJunichi FujiiGeneral / Preventive / Lipids AUAtsushi UenoTokyo Metropolitan University KKKatsuhiko KitanoYokohama National University

Discussion

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Implication

Offers no immediate clinical application; supports consensus on phospholamban structure and extends prior molecular weight estimates.

Key Points

This research aims to elucidate the complete amino acid sequence of canine cardiac phospholamban derived from cDNA.
Isolated cDNA clones specific for phospholamban from a canine cardiac cDNA library.
Deduced amino acid sequence consists of 52 residues, analyzed via sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Phospholamban has a molecular weight of 6,080, consistent with the estimated 6,000 from previous gel electrophoresis.
Identified two distinct domains: a hydrophilic region at the amino terminus and a hydrophobic region at the carboxy terminus.

Structured PICO

Population

Canine cardiac ventricular muscle from adult dog

Intervention

cDNA cloning and amino acid sequencing

Outcome

Complete nucleotide sequence of cDNA and deduced amino acid sequence of phospholamban

The study provides the complete cDNA-derived amino acid sequence of canine cardiac phospholamban, revealing its two-domain structure essential for regulating the sarcoplasmic reticulum calcium pump.

Cite This Study

Fujii et al. (1987) studied this question.

synapsesocial.com/papers/6a135f8371369867e4937d87 https://doi.org/10.1172/jci112799

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Complete complementary DNA-derived amino acid sequence of canine cardiac phospholamban.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Complete complementary DNA-derived amino acid sequence of canine cardiac phospholamban.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

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