What question did this study set out to answer?

To determine whether the catalytic subunit of mu-calpain undergoes autolysis as direct evidence of its proteolytic activation during global cerebral ischemia.

January 1, 1996Journal of Neurochemistry

Brain μ‐Calpain Autolysis During Global Cerebral Ischemia

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To determine whether the catalytic subunit of mu-calpain undergoes autolysis as direct evidence of its proteolytic activation during global cerebral ischemia.
Induced global cerebral ischemia in rabbits using cardiac arrest durations of 0, 5, 10, and 20 minutes.
Electrophoresed rabbit brain homogenates and immunoblotted them using a monoclonal antibody specific to the 80-kDa mu-calpain catalytic subunit.
The average density of the intact 80-kDa subunit decreased by 25 ± 4% (p = 0.008) at 10 minutes and by 28 ± 9% (p = 0.004) at 20 minutes of cardiac arrest.
The average density of the 78-kDa autolyzed fragment increased by 111 ± 50% (p = 0.02) after 20 minutes of cardiac arrest, with no significant change observed in the 76-kDa band.

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Implication

Preclinical study demonstrates mu-calpain catalytic subunit autolysis in rabbit brain tissue, indicating direct enzymatic activation during global cerebral ischemia.

Cite This Study

Neumar et al. (1996) studied this question.

synapsesocial.com/papers/6a14f5c5ecbae6505d9acd12 https://doi.org/10.1046/j.1471-4159.1996.66010421.x

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Implication

Preclinical study demonstrates mu-calpain catalytic subunit autolysis in rabbit brain tissue, indicating direct enzymatic activation during global cerebral ischemia.

Brain μ‐Calpain Autolysis During Global Cerebral Ischemia

Key Points

Discussion

Implication

Cite This Study

Brain μ‐Calpain Autolysis During Global Cerebral Ischemia

Key Points

Discussion

Implication

Cite This Study

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