Key result
Single strand RNA probes derived from a cDNA copy of the HCV229E nucleocapsid protein gene specifically hybridize to HCV229E RNA, detecting approximately 50 pg of intracellular viral RNA.
Why the study?
Can single strand RNA probes derived from a cDNA copy of the HCV229E nucleocapsid protein gene detect intracellular viral RNA?
Can single strand RNA probes derived from a cDNA copy of the HCV229E nucleocapsid protein gene detect intracellular viral RNA?
The development of a specific nucleic acid probe for HCV229E enables the detection of minute amounts of viral RNA, providing a basis for routine diagnostic applications.
Answers are grounded in this paper and cite their sources.
May support nucleic acid detection of HCoV-229E; leaves open clinical diagnostic validation.
A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg of intracellular viral RNA can be readily detected. The application of nucleic acid hybridization as a routine procedure for the diagnosis of HCV229E infection is discussed.
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Myint et al. (1990) studied Human coronavirus 229E infection. Single strand RNA probes derived from cDNA copy of HCV229E nucleocapsid protein gene was evaluated on Detection of intracellular viral RNA. Single strand RNA probes derived from a cDNA copy of the HCV229E nucleocapsid protein gene specifically hybridize to HCV229E RNA, detecting approximately 50 pg of intracellular viral RNA.
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