Key result
In hybrid myotubes, a fusion protein encoded by a single transfected nucleus was not confined to its nucleus of origin but was restricted to nearby nuclei.
Population
C2 myoblasts and hybrid multinucleated myotubes
Design
Preclinical
Authors
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Indicates localized nuclear domains in multinucleated fibers; leaves open relevance to cardiac myocyte gene expression.
In multinucleated myotubes, proteins encoded by a single nucleus can transfer to nearby nuclei, indicating a localized domain of influence rather than strict confinement or global distribution.
Ralston et al. (1989) studied Muscle fiber gene expression. Transfection of C2 myoblasts with a nuclear-targeted reporter protein was evaluated on Distribution of the reporter protein in hybrid myotubes. In hybrid myotubes, a fusion protein encoded by a single transfected nucleus was not confined to its nucleus of origin but was restricted to nearby nuclei.
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