Key result
Digital imaging of fura-2 fluorescence in isolated rat heart cells revealed distinct subcellular patterns of cytoplasmic calcium concentration associated with different cellular behaviors.
The observed cellular and subcellular heterogeneity of [Ca2+]i in isolated cells indicates that experiments performed on suspensions of cells should be interpreted with caution.
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Subcellular [Ca2+]i heterogeneity in isolated rat cardiomyocytes warrants caution interpreting cell-suspension data; leaves open in vivo relevance.
Wier et al. (1987) studied Isolated rat heart cells. Digital imaging of calcium indicator signals (fura-2 fluorescence) was evaluated on Subcellular patterns of cytoplasmic calcium ion concentration ([Ca2+]i). Digital imaging of fura-2 fluorescence in isolated rat heart cells revealed distinct subcellular patterns of cytoplasmic calcium concentration associated with different cellular behaviors.
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