Erythropoietin From the Kidney Cells Cultured in Vitro

1) The kidney cells and bone marrow cells were cultured together in the same culture tube containing 59Fe, and Heme 59Fe synthesis was observed in vitro. When they were cultured in anoxic state, Heme 59Fe synthesis was increased. And when the serum was not used in the culture medium, this response was not observed.2) The parabiotic culture with other cells were studied as a control; The liver cells or spleen cells and bone marrow cells were cultured together in anoxic state, however, Heme 59Fe synthesis was not increased.3) Cobalt chloride was added in the cultured medium, there is no signifi-cant elevation of Heme 59Fe synthesis even in the parabiotic culture of the bone marrow cells and kidney cells.4) Cortex of kidney produces erythropoietin in anoxic state.