Key result
Truncations in recombinant cardiac myosin binding protein C dramatically altered its phosphorylation state compared to the full-length protein, as revealed by top-down high-resolution mass spectrometry.
Truncations in recombinant cMyBP-C dramatically alter its phosphorylation state, indicating that truncated proteins may not accurately reflect the structure and function of the full-length protein in vitro.
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Truncated cMyBP-C models may misrepresent full-length phosphorylation; leaves open validity of recombinant truncations for cardiac protein studies.
Ge et al. (2009) studied this question. Truncation of recombinant cMyBP-C vs. Full-length recombinant cMyBP-C was evaluated on Protein phosphorylation state. Truncations in recombinant cardiac myosin binding protein C dramatically altered its phosphorylation state compared to the full-length protein, as revealed by top-down high-resolution mass spectrometry.
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