An exonuclease III-assisted CHA dual-cycle amplification strategy for quencher-free fluorescent detection of miRNA

Shi-Qian Liao; Jia-Hao Liu; Xin-Yue Liu; Yiting Liu; Chunguang Yang; Zhangrun Xu

doi:10.1039/d6ay00629a

January 1, 2026Analytical Methods

An exonuclease III-assisted CHA dual-cycle amplification strategy for quencher-free fluorescent detection of miRNA

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Key Points

This research aims to develop a sensitive method for detecting miRNA biomarkers essential for cancer diagnosis.
Implemented a quencher-free fluorescent detection approach
Utilized exonuclease III-assisted circular hybridization amplification (CHA)
Optimized conditions for improved sensitivity and specificity
Showed significant enhancement in detection sensitivity of miRNA compared to conventional methods
Achieved a detection limit of x fM, indicating high sensitivity
Demonstrated robust performance across varying miRNA concentrations

Implication

Demonstrates enhanced detection of miRNA biomarkers, suggesting improved cancer diagnosis methods.

Abstract

MicroRNAs (miRNAs) are crucial biomarkers for cancer diagnosis, but their detection is challenged by low abundance and sequence homology. Herein, we report a simple, sensitive, and quencher-free fluorescent method for...

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Cite This Study

Liao et al. (2026) studied this question.

synapsesocial.com/papers/6a168ac80c924ddd1bd5989f https://doi.org/10.1039/d6ay00629a

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An exonuclease III-assisted CHA dual-cycle amplification strategy for quencher-free fluorescent detection of miRNA

Key Points

Implication

Abstract

Discussion

Cite This Study

An exonuclease III-assisted CHA dual-cycle amplification strategy for quencher-free fluorescent detection of miRNA

Key Points

Implication

Abstract

Discussion

Cite This Study

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