Identification of microsatellite loci in lake sturgeon, Acipenser fulvescens, and their variability in green sturgeon, A. medirostris

From (CATC) n , (GATA) n , (AAAC) n , and (CA) n –enriched libraries for the lake sturgeon Acipenser fulvescens , 254 primer pairs were developed. These primer pairs resulted in the identification of 128 microsatellite loci in either A. fulvescens or A. medirostris . Polymorphic loci were identified in both sturgeon species for 48 of the primer pairs and 14 of the primer pairs amplified polymorphic loci only in A. medirostris . Most of the identified loci appear to be tetrasomic (79.1% in A. fulvescens and 64.5% in A. medirostris ). These results offer estimates of the degree of diploidization in each of these species.