September 25, 1992Science
Interactions of Small Nuclear RNA's with Precursor Messenger RNA During in Vitro Splicing
Key result
Psoralen crosslinking revealed that U1, U2, U5, and U6 small nuclear RNAs interact with precursor messenger RNA during in vitro splicing, delineating a temporal pathway for spliceosome action.
Population
HeLa nuclear extract and adenovirus precursor messenger RNA
Design
Preclinical
Authors
Discussion
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Member takes
Implication
In vitro spliceosome mapping; leaves open in vivo validation and relevance to cardiovascular splicing disorders.
Structured PICO
P
Population
HeLa nuclear extract and adenovirus precursor messenger RNA
I
Intervention
Psoralen crosslinking
O
Outcome
Interactions of small nuclear RNAs with precursor messenger RNA during in vitro splicing
The study maps the interactions and temporal pathway of small nuclear RNAs with precursor messenger RNA during in vitro splicing, suggesting the 5' splice site is defined in several steps prior to the first cleavage event.
Cite This Study
Wassarman et al. (1992) studied In vitro RNA splicing. Psoralen crosslinking was evaluated on RNA interactions during splicing. Psoralen crosslinking revealed that U1, U2, U5, and U6 small nuclear RNAs interact with precursor messenger RNA during in vitro splicing, delineating a temporal pathway for spliceosome action.