Key result
Psoralen crosslinking revealed that U1, U2, U5, and U6 small nuclear RNAs interact with precursor messenger RNA during in vitro splicing, delineating a temporal pathway for spliceosome action.
The study maps the interactions and temporal pathway of small nuclear RNAs with precursor messenger RNA during in vitro splicing, suggesting the 5' splice site is defined in several steps prior to the first cleavage event.
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In vitro spliceosome mapping; leaves open in vivo validation and relevance to cardiovascular splicing disorders.
Wassarman et al. (1992) studied In vitro RNA splicing. Psoralen crosslinking was evaluated on RNA interactions during splicing. Psoralen crosslinking revealed that U1, U2, U5, and U6 small nuclear RNAs interact with precursor messenger RNA during in vitro splicing, delineating a temporal pathway for spliceosome action.
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