Key result
NMR spectroscopy revealed that the highly conserved pentaloop of SARS-CoV SL2 adopts a canonical CUYG tetraloop fold with the 3' nucleotide flipped out of the stack.
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The study reveals the canonical CUYG tetraloop fold structure of the SARS-CoV SL2, providing structural context for its function in viral replication.
The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is regulated by specific viral genome sequences within 5'- and 3'-untranslated regions (5'-UTR and 3'-UTR). Here we report the solution structure of 5'-UTR derived stem-loop 2 (SL2) of SARS-CoV determined by NMR spectroscopy. The highly conserved pentaloop of SL2 is stacked on 5-bp stem and adopts a canonical CUYG tetraloop fold with the 3' nucleotide (U51) flipped out of the stack. The significance of this structure in the context of a previous mutagenesis analysis of SL2 function in replication of the related group 2 coronavirus, mouse hepatitis virus, is discussed.
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Lee et al. (2011) studied SARS coronavirus. NMR spectroscopy revealed that the highly conserved pentaloop of SARS-CoV SL2 adopts a canonical CUYG tetraloop fold with the 3' nucleotide flipped out of the stack.
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