Osteopontin-loaded nanoarchaeosomes for enhanced osteogenesis and bone regeneration in osteoporotic zebrafish models

studies demonstrated high biocompatibility in zebrafish embryos, along with increased bone count, mineral density, and regenerative capacity in both fin-fracture and amputation models. Furthermore, zebrafish models of osteoporosis treated with NAO showed improved Ca : P ratios, enhanced mineralization, and upregulation of key osteogenic genes, indicating robust bone regeneration. Collectively, these findings establish osteopontin-loaded nanoarchaeosomes as an efficient nanoplatform capable of sustaining osteogenic signaling and accelerating bone repair, offering a therapeutic strategy to overcome the limitations of current osteoporosis treatment.