Key result
SHG and TPEF microscopy revealed three distinct sarcomeric patterns in skeletal muscle, with a single peak per mature sarcomere in 85% of cases and double peak or mini sarcomeres in the remaining 15%.
SHG microscopy reveals three distinct sarcomeric patterns in skeletal muscle, which may indicate mature sarcomeres, regions of proteolysis, or sarcomeric assembly.
May enable label-free sarcomere assessment in animal models; leaves open translation to human muscle disease.
We have extensively characterized the sarcomeric SHG signal as a function of animal species (rat versus xenopus), age (adult versus larval) and tissue preparation (fixed or fresh) and we found that the main feature of this signal is a single peak per mature sarcomere (about 85% of all sarcomeres). The remaining (15%) was found to be either double peak per mature sarcomere or mini sarcomeres (half of a sarcomere) using alpha-actinin immuno detection of the Z-band. The mini sarcomeres are often found in region of pitchfork-like SHG pattern. We suggest that double peak SHG pattern could indicate regions of sarcomeric proteolysis whereas pitchfork-like SHG pattern could reveal sarcomeric assembly.
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Recher et al. (2009) studied Skeletal muscle sarcomeres. SHG and TPEF Microscopy was evaluated on Sarcomeric SHG signal patterns. SHG and TPEF microscopy revealed three distinct sarcomeric patterns in skeletal muscle, with a single peak per mature sarcomere in 85% of cases and double peak or mini sarcomeres in the remaining 15%.
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