Programmable wide-range pH gradients for NMR titrations: application to antibody–drug conjugate linker group modifications

This is the authors' abstract. We don't add key points for this paper.

Tuneable pH gradients are generated within NMR tubes using buffer components enabling extraction of the p K a of polyprotic molecules by chemical shift imaging. Knowledge of p K a informs the design of vinylpyridine antibody drug conjugate linkers.