Transcriptomic profile of cationic channels in human pulmonary arterial hypertension

Figure 1. Channel gene expression profile analysis in pulmonary arterial hypertension. (A) Vulcano plot [log fold change against − log (adjusted p value)] showing the changes in mRNA transcripts for all identified genes (blue) and for cationic channel encoding genes (red). The vertical lines correspond to twofold up and down, respectively, and dots above the horizontal dotted line indicate adjusted p < 0.05. (B) Families and subfamilies of mRNAs encoding the pore forming alpha subunits of cationic channels identified in the control samples, (C) 3D principal component analysis performed with the 143 channel encoding mRNAs. (D) Regression analysis of the changes (fold change vs control) in the different subgroups of PAH: idiopathic PAH (IPAH), connective tissue disease associated PAH (CTD-PAH) and congenital heart disease PAH (CHD-PAH).

Figure 2. Relative abundance of ion channel encoding genes in the control lungs. Bars represent means ± SEM (n = 10). Arrows indicate significantly (adjusted p < 0.05) dysregulated channels in PAH: dark green for > twofold downregulation, light green for < twofold downregulation and red for > twofold upregulation.

Figure 3. Heat map of genes encoding cationic channel that displayed significant difference (adjusted p < 0.05) in expression.

Figure 4. Vulcano plot [log fold change against − log (adjusted p value)] showing the changes in mRNA transcripts for cationic channel encoding genes (A) K+, B) Ca2+, C) Na+, D) TRP and E) CNG/HCN superfamilies. The vertical lines correspond to twofold up and down, respectively, and dots above the horizontal dotted line indicate adjusted p < 0.05.