HF Cleavage and Deprotection Procedures for Peptides Synthesized Using a Boc/Bzl Strategy

On completion of chemical synthesis of the peptide chain, the final step requires the removal from the solid-phase support and liberation of the protected side chains of the trifunctional amino acids (1). Many different approaches to this problem have been established, but the procedure most widely used for all Boc/Bzl-based peptides has been treatment with liquid HF.