What question did this study set out to answer?

To investigate whether the cell cycle phase of recipient cells influences the transduction efficiency and integration of adeno-associated virus vectors.

September 13, 1994Proceedings of the National Academy of SciencesOpen Access

Adeno-associated virus vectors preferentially transduce cells in S phase.

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Authors

DRDavid W. RussellLewis University AMA. Dusty MillerBrigham Young University IAIan E. AlexanderThe University of Sydney

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Implication

Experimental study demonstrates a 200-fold higher transduction frequency for S phase primary human fibroblasts, suggesting cell division significantly enhances adeno-associated virus gene therapy.

Key Points

To investigate whether the cell cycle phase of recipient cells influences the transduction efficiency and integration of adeno-associated virus vectors.
Infected stationary and dividing primary human fibroblast cultures with adeno-associated virus vectors encoding alkaline phosphatase and neomycin phosphotransferase.
Assessed vector genome persistence, transduction rates across cell cycle stages, and chromosomal integration patterns following growth stimulation.
Transduction frequency was approximately 200 times higher in S phase cells than in non-S phase cells.
Neither S phase nor mitosis was strictly essential for transduction, as single-stranded vector genomes persisted in stationary cultures and were recruited for transduction once division was stimulated.
Stable transductants exhibited randomly integrated vector sequences within the host genome.

Cite This Study

Russell et al. (1994) studied this question.

synapsesocial.com/papers/6a197f7eb71d9c8593891d02 https://doi.org/10.1073/pnas.91.19.8915

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Implication

Experimental study demonstrates a 200-fold higher transduction frequency for S phase primary human fibroblasts, suggesting cell division significantly enhances adeno-associated virus gene therapy.

Adeno-associated virus vectors preferentially transduce cells in S phase.

Discussion

Member takes

Implication

Key Points

Cite This Study

Adeno-associated virus vectors preferentially transduce cells in S phase.

Discussion

Member takes

Implication

Key Points

Cite This Study

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