Activation of Adipose Tissue Lipase by Skeletal Muscle Cyclic Adenosine 3',5'-Monophosphate-stimulated Protein Kinase

Abstract A purified rabbit skeletal muscle adenosine 3',5'-monophosphate (cyclic AMP)-stimulated protein kinase enhanced lipolytic activity in adipose tissue homogenates. This effect required the presence of cyclic AMP and ATP and was completely blocked by a protein inhibitor of cyclic AMP-stimulated protein kinases. It is inferred that this effect is due to the phosphorylation and activation of a lipase in a system analogous to that involved in the activation of muscle glycogen phosphorylase.