What question did this study set out to answer?

To evaluate whether in vivo transfer and local expression of a human epidermal growth factor plasmid can accelerate partial-thickness wound healing.

December 6, 1994Proceedings of the National Academy of SciencesOpen Access

In vivo transfer and expression of a human epidermal growth factor gene accelerates wound repair.

View Full Paper

Ask AI

Bookmark

Authors

CAChristoph AndreeDüsseldorf University Hospital WSWilliam F. SwainRoyal Hampshire County Hospital CPClive PageKing's College London

Discussion

Member takes

Implication

In vivo experimental study demonstrates accelerated wound closure in porcine skin, indicating that targeted epidermal growth factor gene delivery enhances tissue repair.

Key Points

To evaluate whether in vivo transfer and local expression of a human epidermal growth factor plasmid can accelerate partial-thickness wound healing.
Transferred a human epidermal growth factor (hEGF) expression plasmid into porcine partial-thickness wound keratinocytes via particle-mediated DNA transfer (Accell).
Applied a sealed, fluid-filled external wound chamber to shield wounds, isolate exogenous DNA and expressed peptides, and sample wound fluid over a 10-day period.
Wounds bombarded with the hEGF plasmid demonstrated a 190-fold increase in EGF concentration, declining from 200 pg/ml to 25 pg/ml over the initial 5 days and remaining detectable across 10 days.
Treated wounds achieved re-epithelialization 20% (2.1 days) earlier than controls, and plasmid DNA remained detectable by polymerase chain reaction for at least 30 days.

Cite This Study

Andree et al. (1994) studied this question.

synapsesocial.com/papers/6a19beaa05af093a17f6801a https://doi.org/10.1073/pnas.91.25.12188

View Full Paper

Ask AI

Bookmark

December 6, 1994Proceedings of the National Academy of SciencesOpen Access

In vivo transfer and expression of a human epidermal growth factor gene accelerates wound repair.

View Full Paper

Ask AI

Bookmark

Authors

CAChristoph AndreeDüsseldorf University Hospital WSWilliam F. SwainRoyal Hampshire County Hospital CPClive PageKing's College London

Discussion

Member takes

Implication

In vivo experimental study demonstrates accelerated wound closure in porcine skin, indicating that targeted epidermal growth factor gene delivery enhances tissue repair.

Key Points

To evaluate whether in vivo transfer and local expression of a human epidermal growth factor plasmid can accelerate partial-thickness wound healing.
Transferred a human epidermal growth factor (hEGF) expression plasmid into porcine partial-thickness wound keratinocytes via particle-mediated DNA transfer (Accell).
Applied a sealed, fluid-filled external wound chamber to shield wounds, isolate exogenous DNA and expressed peptides, and sample wound fluid over a 10-day period.
Wounds bombarded with the hEGF plasmid demonstrated a 190-fold increase in EGF concentration, declining from 200 pg/ml to 25 pg/ml over the initial 5 days and remaining detectable across 10 days.
Treated wounds achieved re-epithelialization 20% (2.1 days) earlier than controls, and plasmid DNA remained detectable by polymerase chain reaction for at least 30 days.

Cite This Study

Andree et al. (1994) studied this question.

synapsesocial.com/papers/6a19beaa05af093a17f6801a https://doi.org/10.1073/pnas.91.25.12188

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark