Electrochemical Monitoring of Hydrogen Sulfide Release from Single Cells

Real‐time and in situ monitoring of H 2 S in biological systems has become crucial, but faces big challenges as H 2 S has been identified as an important endogenous gas signaling molecule. Herein, we prepare a poly(3,4‐ethylenedioxythiophene) (PEDOT)‐modified nanoporous gold (NPG) microelectrode for the electrochemical detection of H 2 S. The NPG framework possesses excellent conductivity, a 3D porous structure, and a large specific area. The PEDOT sensing film effectively retards electrode fouling during the detection, which ensures a stable response towards H 2 S over the range of interest. The synergetic properties of the NPG scaffold and the PEDOT sensing films endow the microelectrode with high sensitivity and a fast response, thus allowing H 2 S release from single cells to be monitored. This work represents the first step toward real‐time electrochemical monitoring of H 2 S from single cells, thereby potentially facilitating future studies to better understand the function of H 2 S in biological and pathological processes.