Peripancreatic Adipose Tissue Remodeling and Inflammation during High Fat Intake of Palm Oils or Lard in Rats

Figures

• 

  Figure 1. Adipocyte size and macrophage infiltration in epididymal white adipose tissue (eWAT). (A) Representative microphotographs of haematoxylin staining (×200; scale bar = 20 µm); (B) quantitative analysis of adipocyte area (µm2); (C) relative frequency of adipocyte area (%); (D) quantitative analysis of immunohistochemistry for CD68 (number of positive cells/mm2 of adipose tissue). Data are shown as means ± SEM, n = 4–7 animals per group. * p < 0.05 vs. control diet.

• 

  Figure 2. Adipocyte size and macrophage infiltration in peripancreatic adipose tissue (pWAT). (A) Representative microphotographs of haematoxylin staining (×200; scale bar = 20µm); (B) quantitative analysis of adipocyte area (µm2); (C) relative frequency of adipocyte area (%); (D) quantitative analysis of immunohistochemistry for CD68 (number of positive cells/mm2 of adipose tissue). Inset presents an example of CD68 staining in refined PO. Data are shown as means ± SEM, n = 4–7 animals per group. * p < 0.05 vs. control diet.

• 

  Figure 3. Lipid inclusions in periaortic brown adipose tissue (AoBAT). (A) Representative microphotographs of haematoxylin staining (×200; scale bar = 20 µm); (B) quantitative analysis of lipid inclusions as % of total tissue area; (C) box plot displaying the distribution of lipid inclusions. Quantification of lipid inclusion was realised in 10–30 fields per rat. Data are shown as means ± SEM, n = 7 animals per group. * p < 0.05 vs. control diet.

• 

  Figure 4. Impact of high intake of palm oils and lard on isoprostanoid concentration in peripancreatic adipose tissue (pWAT). (A) Quantification of isoprostanoids with a trans configuration: 15(RS)-15-F2t-IsoP and 5(RS)-5-F2t-IsoP (fg/adipocyte). (B) Quantification of 5(RS)-5-F2c-IsoP, an isoprostanoid with a cis configuration (fg/adipocyte). (C) Quantification of the isofuran 7(RS)-ST- Δ8-11-dihomo-IsoF (fg/adipocyte). Isoprostanoids were assayed from 200 mg of adipose tissue per rat. Data are shown as means ± SEM, n = 6–7 animals per group. * p < 0.05 or ** p < 0.01 vs. control diet.