What question did this study set out to answer?

To determine the subunit composition of plant eukaryotic initiation factor 3 (eIF3) and characterize its evolutionary relationship to mammalian and yeast eIF3 complexes.

January 1, 2001Journal of Biological ChemistryOpen Access

Plant Initiation Factor 3 Subunit Composition Resembles Mammalian Initiation Factor 3 and Has a Novel Subunit

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To determine the subunit composition of plant eukaryotic initiation factor 3 (eIF3) and characterize its evolutionary relationship to mammalian and yeast eIF3 complexes.
Purified wheat eIF3 subunits and performed peptide sequencing to correlate plant subunits with known mammalian and Saccharomyces cerevisiae homologs.
Screened and identified seven new Arabidopsis thaliana cDNAs encoding eIF3 subunits based on wheat peptide sequences.
Purified and biochemically characterized A. thaliana eIF3 to evaluate subunit composition and functional activity relative to wheat eIF3.
Plant eIF3 shares 10 of its 11 subunits with mammalian eIF3, closely matching mammalian subunit architecture rather than the divergent yeast complex.
Identified a novel, plant-specific subunit within the eIF3 complex that is absent in both mammalian and S. cerevisiae complexes.
Isolated and sequenced seven novel A. thaliana cDNAs corresponding to eIF3 complex subunits.

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Implication

Comparative biochemical analysis reveals plant eIF3 shares ten subunits with mammals and contains a novel subunit, suggesting conserved evolutionary pathways between plants and mammals.

Cite This Study

Burks et al. (2001) studied this question.

synapsesocial.com/papers/6a19e03205af093a17f6afd7 https://doi.org/10.1074/jbc.m007236200

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Implication

Comparative biochemical analysis reveals plant eIF3 shares ten subunits with mammals and contains a novel subunit, suggesting conserved evolutionary pathways between plants and mammals.

Plant Initiation Factor 3 Subunit Composition Resembles Mammalian Initiation Factor 3 and Has a Novel Subunit

Key Points

Discussion

Implication

Cite This Study

Plant Initiation Factor 3 Subunit Composition Resembles Mammalian Initiation Factor 3 and Has a Novel Subunit

Key Points

Discussion

Implication

Cite This Study

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