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January 1, 1993Nippon Ishinkin Gakkai ZasshiOpen Access

Polymerase Chain Reaction for the Diagnosis of Candidiasis: Detection of Candida albicans DNA in Cerebrospinal Fluid.

Ikuyo Kanaizuka Yasuyuki Sugita Hiroshi Nakajima

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Abstract

Candida albicansのsecretory aspartate proteinase遺伝子の塩基配列中にpolymerase chain reaction (PCR) 法によるDNAの増幅領域を設定し, C. albicansとC. stellatoideaでは明らかな標的領域の増幅が確認された. この方法でカンジダ性髄膜炎の患者髄液からDNAの増幅を行い, オリゴヌクレオチドプローブを用いたサザンプロットハイブリダイゼーションで増幅DNAの特異性を確認した. こうして迅速で高感度なPCR法がカンジダ性髄膜炎の早期診断に有用であることが示された.

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Kanaizuka et al. (1993) studied this question.

synapsesocial.com/papers/6a19eac72f689499b5f6a7d8 https://doi.org/10.3314/jjmm.34.13

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