⌘+K

Pulse Explore Communities Researchers Journals

Home Communities Explore

May 28, 2026International Journal of Molecular SciencesOpen Access

A Simple Method to Produce Recombinant Mammalian Serum Albumins in Escherichia coli Preserving Intact Antigenic Properties

Anna S. Dolgova Anna S. Cherkashina Alexander I. Shcherbakov Kseniya A. Lashkevich Irina Goptar Irina P. Lisyukova Anna E. Sudina Olga A. Stukolova

View Full Paper

Ask AI

Bookmark

Share

Key Points

The aim is to develop a simple method to produce soluble and functional recombinant feline and canine serum albumins in E. coli.
Used E. coli strain BL21 (DE3) for expression.
Removed the 18-amino acid signal peptide from the N-terminus of albumins.
Tested fusion proteins like MBP, TF, and NusA to enhance solubility and expression.
rFel d 2 and rCan f 3 bound IgE with similar effectiveness to their native counterparts.
Immunoglobulin E binding rates were comparable: 26/33 for rFel d 2 and 27/29 for rCan f 3 using TF, MBP, and NusA.
The fusion strategy and signal peptide removal enabled functional protein production.

No abstract yet

We only have this paper's title and publication details.

Read it at the source→

Implication

Demonstrates enhanced production of functional serum albumins in E. coli, indicating a new method for allergen research.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Dolgova et al. (2026) studied this question.

synapsesocial.com/papers/6a1a7fef0307b7850943221c https://doi.org/10.3390/ijms27114853

View Full Paper

Ask AI

Bookmark

Share

Keep reading

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1Albumin Binding as a General Strategy for Improving the Pharmacokinetics of ProteinsOriginal Research · 2002 · 508 citations
2A specific albumin receptor mediates antigen delivery and the induction of B cell responses in secondary lymphoid organs 2259364Original Research · 2026
3Production of antibodies and antibody fragments containing non-natural amino acids in <i>Escherichia coli</i>2024 · 7 citations
4B-105 A New Recombinant Human Albumin Produced in Thermothelomyces heterothallica (C1) Demonstrates Comparable Surface Binding to Native Serum Albumin2025 · 1 citations
5Enhanced therapeutic potential of antibody fragment via IEDDA-mediated site-specific albumin conjugation2024 · 8 citations

More related papers

Evaluation of Inhibitory Activity of Novel Monoclonal Antibodies Against Cat Allergen Fel d 7 and Their Application to Analyse Allergen Extracts.2025
B-060 A Recombinant Human Albumin Produced in Thermothelomyces heterothallica (C1) as a High-Purity Alternative for Diagnostic Assays2025
Optimizing the Amino Acid Sequence Enhances the Productivity and Bioefficacy of the RBP-Albumin Fusion Protein2024

More from International Journal of Molecular Sciences

Dual-Metal Oxide on Cellulose Nanofibers for Advanced SupercapacitorsFri,
Lessons from Plant–Microbe Interactions: Concepts for Improving Plant Nutrition and Stress ToleranceFri,