Identification of SNPs Associated With mRNA Expression Differences of the Complement Receptor Type 1‐Like Gene in Landrace Weaned Piglets

OBJECTIVE: The aim of this study is to investigate the association between single nucleotide polymorphisms (SNPs) of porcine complement receptor 1-like (CR1-like) gene and its mRNA expression. METHODS: SNP identification, qRT-PCR absolute quantification, linkage disequilibrium analysis and dual-luciferase reporter assay were performed using 392 forty-day-old Landrace weaned piglets. RESULTS: 28 SNPs were identified, 4 loci were significantly correlated with CR1-like expression (p < 0.05). The c.-1372GA mutation led to the loss of GATA-1 binding site, and significantly reduced promoter transcriptional activity (< P0.01). CONCLUSION: CR1-like gene polymorphisms regulate its expression via disrupting transcription factor binding, which provides a theoretical basis for porcine disease-resistant breeding.