Asymmetric LAMP–gold nanoparticle biosensing for rapid detection of Kenyan tomato leaf curl virus isolates from crude extracts

Q: What does this research mean for the field?

Asymmetric LAMP combined with gold nanoparticle biosensing enables rapid, solution-based visual detection of Kenyan tomato leaf curl virus from crude extracts by generating more ssDNA amplicons for efficient probe hybridization. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Abigarl Ndudzo; Florence Ngonga; Edith K. Avedi; Elijah M. Ateka

doi:10.1039/d6ra04196e

January 1, 2026RSC AdvancesOpen Access

Asymmetric LAMP–gold nanoparticle biosensing for rapid detection of Kenyan tomato leaf curl virus isolates from crude extracts

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Key Points

The aim is to evaluate the effectiveness of asymmetric LAMP combined with gold nanoparticles for detecting tomato leaf curl virus isolates.
Utilized asymmetric LAMP to generate single-stranded DNA (ssDNA) amplicons.
Performed hybridization with DNA probes for detection.
Established stable gold nanoparticle-DNA associations for visual readouts.
Achieved rapid and effective virus detection from crude extracts.
Demonstrated high stability of the AuNP-complementary DNA associations.
Resulted in a clear visual readout for determining virus presence.

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Implication

Demonstrates efficient detection of tomato leaf curl virus in crude extracts, indicating a promising diagnostic tool.

Abstract

Asymmetric LAMP generates more ssDNA amplicons, followed by efficient hybridization with DNA probe, yielding stable AuNP-complementary DNA association for a solution-based visual readout.

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Ndudzo et al. (2026) studied this question.

synapsesocial.com/papers/6a1a820e0307b78509433cd6 https://doi.org/10.1039/d6ra04196e

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Asymmetric LAMP–gold nanoparticle biosensing for rapid detection of Kenyan tomato leaf curl virus isolates from crude extracts

Key Points

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Implication

Abstract

Discussion

Cite This Study

Asymmetric LAMP–gold nanoparticle biosensing for rapid detection of Kenyan tomato leaf curl virus isolates from crude extracts

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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