Insulin relaxes vascular smooth muscle via dual mechanisms involving endothelium-dependent NO release and direct reduction of smooth muscle cytosolic calcium.
To determine the mechanism of the inhibitory effect of insulin on vascular tone, contraction was measured simultaneously with endothelial and smooth muscle cytosolic Ca2+ level (Ca2+i) in the isolated rat aorta. Insulin (200 mU/mL) increased endothelial Ca2+i and decreased resting muscle tone. The removal of endothelium abolished the effects of insulin. In the aorta precontracted with norepinephrine, insulin (3 to 120 mU/mL) induced concentration-dependent inhibition of contraction. The relaxant effect followed the increase in endothelial Ca2+i and decrease in smooth muscle Ca2+i. The relaxant effect was attenuated by removal of endothelium or by the addition of 10(-5) mol/L NG-monomethyl-L-arginine but not by 10(-5) mol/L indomethacin. In the absence of endothelium, the relaxant effect of insulin followed the decrease in smooth muscle Ca2+i. These results suggest that insulin inhibits vascular contraction by dual mechanisms in the isolated rat aorta: (1) Insulin acts on vascular endothelium by increasing endothelial Ca2+i and releasing NO, which decreases smooth muscle Ca2+i and the Ca2+ sensitivity of the contractile elements. (2) Insulin also directly acts on smooth muscle and decreases smooth muscle Ca2+i.
Han et al. (Sun,) studied this question.
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