Enhanced MICP for Soil Improvement and Heavy Metal Remediation: Insights from Landfill Leachate-Derived Ureolytic Bacterial Consortium

This study investigates the potential of microbial-induced calcium carbonate precipitation (MICP) for soil stabilization and heavy metal immobilization, utilizing landfill leachate-derived ureolytic consortium. Experimental conditions identified yeast extract-based media as most effective for bacterial growth, urease activity, and calcite formation compared to nutrient broth and brown sugar media. Optimal MICP conditions, at pH 8–9 and 30 °C, supported the most efficient biomineralization. The process facilitated the removal of Cd2+ (99.10%) and Ni2+ (78.33%) while producing stable calcite crystals that enhanced soil strength. Thermal analyses (thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC)) confirmed the successful production of CaCO3 and its role in improving soil stability. DSC analysis revealed endothermic and exothermic peaks, including a significant exothermic peak at 444 °C, corresponding to the thermal decomposition of CaCO3 into CO2 and CaO, confirming calcite formation. TGA results showed steady weight loss, consistent with the breakdown of CaCO3, supporting the formation of stable carbonates. The MICP treatment significantly increased soil strength, with the highest surface strength observed at 440 psi, correlating with the highest CaCO3 content (18.83%). These findings underscore the effectiveness of MICP in soil stabilization, pollutant removal, and improving geotechnical properties.