A cannula, and a method for its accurate implantation into predetermined brain regions, is described. This technique makes possible the accurate injection of minute quantities of chemicals into specific deep regions of the brain. The behavioral effects and concomitant changes in electrical recordings from subcortical structures induced by the injection of a variety of compounds and brain extracts into selected brain regions of cats, monkeys, and humans are described. The most consistent and dramatic changes were induced with the injection of bovine septal extract, histamine, and atropine.
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Heath et al. (1961) studied this question.
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