EDTA dissociation of reticulocyte polyribosomes liberates globin mRNA as a nucleoprotein. An attempt to compare the proteins forming the mRNA · protein complexes with the initiation factors of protein synthesis is presented here. Two conditions are described under which initiation factors are removed from hemoglobin‐synthesizing ribosomes. One is after treating the polyribosomes with a high salt concentration; the other is by isolating ribosomes from reticulocytes at an advanced state of maturation which have lost their initiation factors. In both cases it is shown that the mRNA remains associated with the ribosomes and with the proteins forming the mRNA · protein. Moreover both mRNA and mRNA · protein are translatable in a heterologous cell‐free system and their translation is stimulated to the same extent by the addition of reticulocyte initiation factors. Initiation factors are therefore different physically and functionally from the proteins of the mRNA · protein.
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Nudel et al. (1973) studied this question.
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