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May 31, 2026Microbiology Spectrum0 citationsOpen Access

Generation of Hermes Insertion Mutants in Prototrophic Candida Glabrata for Nutrient-Limited...

Facile generation of Hermes insertion mutants in prototrophic Candida glabrata for use in nutrient-limited environments

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Authors

AZAnna M. ZaeskeJohns Hopkins UniversityAHAbigail A. HarringtonJohns Hopkins UniversityTNTimothy J. NickelsJohns Hopkins University

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Overview

Randomized trial generates insertion mutants in prototrophic Candida glabrata, indicating improved methods for studying colonization dynamics.

Key Points

  • The aim is to develop a method for generating transposon insertion mutants in Candida glabrata without compromising fitness during colonization in vivo.
  • Introduced a cycloheximide resistance mutation in the RPL28 gene of C. glabrata strains.
  • Generated prototrophic pools of Hermes transposon insertion mutants likely avoiding auxotrophic limitations.
  • Assessed the effects of pdr1∆ mutants in gastrointestinal tract colonization.
  • High insertion densities were achieved in prototrophic C. glabrata strains.
  • Observed correlations between subtelomeric chromatin compaction and genetic variations in the SIR3 gene.
  • pdr1∆ mutants showed moderate fitness defects in the mouse gastrointestinal tract even without antifungals.
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Cite This Study

Zaeske et al. (2026) studied this question.

synapsesocial.com/papers/6a1bd0525783ba022b6fc36dhttps://doi.org/10.1128/spectrum.00848-26
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