Randomized trial examines serum endocan levels in rats after ischemia–reperfusion injury, indicating its potential as a biomarker.
Key Points
The study aims to evaluate serum endocan as a biomarker for cerebral ischemia–reperfusion injury in a rat model.
Sixteen adult male Sprague Dawley rats were randomly assigned to sham or ischemia–reperfusion (I/R) groups.
Serum endocan levels were measured using ELISA at baseline and at 6, 24, and 48 hours post-reperfusion.
Histopathological examination of hippocampal neuronal degeneration was conducted at 48 hours.
Endocan levels were significantly higher in the I/R group compared to the sham group at 6 h (p < 0.005), 24 h (p < 0.001), and 48 h (p < 0.001).
Significant correlation between serum endocan levels at 48 h and hippocampal neuronal degeneration scores (Spearman's ρ = 0.857, p = 0.007).
Histopathological analysis showed greater neuronal degeneration in the I/R group (median score = 2.5) compared to the sham group (median score = 0; p < 0.001).