Standardization, Phytochemical Profiling, Heavy Metal Safety and Microbial Quality Evaluation of Vetrilai Thylam: A Siddha Herbal Oil Formulation

Background: Standardization of Siddha formulations is essential to ensure quality, safety, and reproducibility. Vetrilai Thylam is a traditional herbal oil widely used in Siddha practice. Aim: To evaluate the physicochemical parameters, heavy metal safety, and phytochemical constituents of Vetrilai Thylam. Materials and Methods: The formulation was analyzed using PLIM protocols for ASU drugs. Physicochemical parameters were assessed as per standard procedures. Heavy metal analysis was performed according to WHO guidelines. Phytochemical screening and sterility testing were carried out using standard microbiological and phytochemical methods. Results: The formulation exhibited acceptable physicochemical parameters. Heavy metals were within permissible limits. No microbial growth was observed in sterility testing, confirming the absence of contamination. Phytochemical evaluation indicated the presence of bioactive principles supporting therapeutic potential. Conclusion: The study confirms that Vetrilai Thylam meets safety standards and contains significant bioactive compounds, supporting its therapeutic potential and validating its traditional use.